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. 2021 May 10;19:67. doi: 10.1186/s43141-021-00171-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Molecular analysis of transgenic wheat plants. I Analysis by PCR of the IPT gene (a), BAR gene (b), and GHS gene (c). Lane 1, negative control; lane 2, positive control; lanes 3–8, six independent putative transgenic plants; all plants had the amplified gene as expected. II Analysis by RT-PCR of the BAR gene (a) and GHS gene (b). Lane 1, negative control; lane 2, positive control; lanes 3–8, six independent putative transgenic plants. III Southern blot analysis on T₁ plants derived from transgenic events, indicating the number of copies of each transgenic event