Fig. 3.

GFP-based forward genetic screen reveals mutants with swollen afferent terminals. (a–b) The Tg(neurod1:EGFP)^nl1 transgenic line can be used to visualize the afferent terminals beneath hair cells in the lateral line (a–b, green), while the vital dye FM 4-64 can be used to visualize lateral line hair cells (a, b, magenta). Actr10^nl15 mutants were isolated in a forward genetic screen using Tg(neurod1:EGFP)^nl1 to identify mutants with swollen lateral line afferent terminals (b) (Drerup et al. 2017). Hair cells in these mutant do not label with FM 4-64. Arrowheads indicate swellings in b. Images were taken at 5 dpf at × 63 magnification. Scale bar in b = 5 µm