Fig. 6.

5-HPF extends the mean lifespan of C. elegans by altering the autophagy pathway. a No significant augmentation in the mean lifespan of the mutant was observed in unc-51 (BE150) mutant (2.17%, p = 0.446). b 50 μM 5-HPF-treated bec-1 (VC424) mutant (− 0.36%, p = 0.864) failed to extend the mean lifespan of worm in comparison with the control group. c Representative image of localization of lgg-1::gfp puncta in autophagic membranes treated with 50 μM 5-HPF and control. d Graphical representation of localization of lgg-1::gfp puncta in autophagic membranes treated with 50 μM 5-HPF and control. e 5-HPF treatment significantly upregulated the fold change in the mRNA expression level of genes bec-1 and lgg-1. The images were quantified by determining the average pixel intensity in each worm using ImageJ software (NIH). The data were analyzed using the Kaplan–Meier survival analysis, two-way ANOVA, and independent t test wherever applicable in GraphPad Prism version 5. Differences between the data were considered significant at p ≤ .05 (*p value < 0.05 and ***p value < 0.001; scale bar = 200 μm, N = 30/group)