FIGURE 4.

Impact of ripening mutations on the CNR, NOR, and RIN transcription factors. Relative gene expression of the three transcription factors across ripening in the wild type (WT), Cnr, nor, and rin genotypes measured by quantitative reverse transcription (qRT)-PCR. The measurements were done with fruit (n = 6) collected at 37 days post anthesis (dpa), equivalent to the mature green stage (MG), 45 dpa, equivalent to the turning stage (T), 50 dpa, equivalent to the red ripe stage (RR), and 57 dpa, equivalent to the overripe (OR) stage. Error bars represent standard error between biological replicates of each sample. Letters indicate significant differences among genotypes and ripening stages calculated by ANOVA and Tukey HSD (P ≤ 0.05).