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. 2021 Mar 19;124(10):1670–1679. doi: 10.1038/s41416-021-01297-x

Fig. 1. Generation and functional validation of LDHA knockdown constructs.

Fig. 1

a, b Representative Western blot analysis of clones for LDHA, LDHB and β-actin. Hth83 and 22A cell lines were infected with lentivirus containing shLDHA constructs. Clones were isolated from the parental population and expanded. LDHA was suppressed by DOX for 48 h. c Following exposure to DOX for 24–96 h, LDHA and β-actin levels were evaluated using Western blot. Activation of shLDHA constructs suppressed LDHA protein levels within 24 h of exposure. d LDH activity continued to drop significantly at both 24 and 96 h after activation, but lactate levels equilibrated at 96 h after activation. All experiments were performed at least triplicate, and every condition was tested at least in triplicate. Error bars represent standard deviation. *P < 0.05 compared with control condition.