Fig. 4. LDHA is required for maximal tumour proliferation and survival.

Hth83 (a) and 22A (b) clones demonstrated significant tumour growth inhibition following LDHA suppression at day 5 post injection, compared with the control, which is consistent with relative LDHA activity levels. c Western blots demonstrating suppression of LDHA protein levels in tumours following treatment with DOX. d LDH activity was significantly decreased following construct activation. e Withdrawal of DOX to restore LDHA expression resulted in tumour growth restoration. f Suppression of LDHA significantly prolonged median survival time. Data for a, b, and e are presented as means with error bars representing standard deviations. *P < 0.05 compared with control condition.