Table 1.

Primer sequences and characteristics of PCR amplifications in I. germanica.

Gene	Description	Accession numbers	Arabidopsis ortholog	Primer sequence (forward/reverse)	Product size (bp)	Primers TM (℃)	Amplification efficiency (%)	R2
IgEF1α	Elongation factor 1 alpha	MN602628	At1G07940	5′-ACCATACCAGGCTTGATAACTCC-3′ 5′-AGACTGGTACAAGGGTCCTACTCTC-3	171	59.4/59.5	100.63%	0.9995
IgGAPDH	Glyceraldehyde-3-phosphate dehydrogenase	MN602629	At2G24270	5′-TTTGCTGACGACTCGGACAC-3′ 5′-CTTGGATTTGGTTGCTGCTAAT-3′	204	59.1/59.1	98.47%	0.9998
IgACT6	Actin6	MN602630	At2G31200	5′-TGCTGCTTTGATTGCGTGT-3′ 5′-AGCTCCATACAATCGACTCAGG-3′	107	58.2/58.6	98.74%	0.9985
IgUBQ	Ubiquitin	MN602631	At4G05320	5′-GATGGTCGCACACTTGCTGA-3′ 5′-GGAGCCTGAGAACAAGATGGA-3′	295	59.9/59.1	101.74%	0.9997
IgUBC	Ubiquitin-protein ligase	MN602632	At5G53300	5′-CCTCCCTTTCCAATCGCTAA-3′ 5′-AGGTGCTGCTGTCCATCTGTT-3′	162	59.5/59.5	101.67%	0.9997
IgEF1β	Elongation factor 1β	MN602633	At5G19510	5′-TTGGAGGAGACCGTTCGC-3′ 5′-TCATTGGCAGGCTCAACAGT-3′	173	58.8/58.7	100.64%	0.9995
IgPGK	Phosphoglycerate kinase	MN602634	At1G56190	5′-GTTGTGCCAGCGTCTGAAAT-3’ 5′-ACCTCGGCTACTCCCACTTT-3′	263	58.4/58.0	101.19%	0.9994
IgTUB	Beta-tubulin	MN602635	At1G64740	5′-GTTTGACTTCCAGTTTGGTTGTG-3′ 5′-GCAAAACAAACACCCGCTTA-3′	274	59.0/59.0	98.77%	0.9984
IgF3H	Flavanone 3-hydroxylase	MN602636	At3G51240	5′-GGTTCATTGTCTCCAGCCATC-3′ 5′-ATTGCTTCGGAGAGGACCC-3′	202	59.2/58.8	98.70%	0.9991