Table 3.
Expression stability analysis of 8 candidate reference genes calculated using BestKeeper software.
| Rank | ‘00246’ | ‘2010200’ | ‘Elizabeth’ | Total | ||||
|---|---|---|---|---|---|---|---|---|
| Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | Gene | CV ± SD | |
| 1 | IgGAPDH | 0.33 ± 0.08 | IgTUB | 0.87 ± 0.20 | IgGAPDH | 0.66 ± 0.16 | IgGAPDH | 0.89 ± 0.21 |
| 2 | IgUBC | 0.62 ± 0.12 | IgACT6 | 1.58 ± 0.36 | IgUBC | 0.92 ± 0.19 | IgUBC | 1.39 ± 0.28 |
| 3 | IgTUB | 1.25 ± 0.27 | IgGAPDH | 1.86 ± 0.45 | IgTUB | 2.96 ± 0.66 | IgTUB | 2.91 ± 0.65 |
| 4 | IgEF1β | 1.74 ± 0.39 | IgUBC | 2.58 ± 0.53 | IgEF1ɑ | 3.52 ± 0.76 | IgACT6 | 2.98 ± 0.68 |
| 5 | IgACT6 | 2.00 ± 0.47 | IgPGK | 4.63 ± 0.97 | IgPGK | 3.67 ± 0.84 | IgPGK | 3.98 ± 0.92 |
| 6 | IgEF1ɑ | 2.62 ± 0.57 | IgEF1β | 4.70 ± 1.06 | IgACT6 | 3.71 ± 0.84 | IgEF1ɑ | 4.07 ± 0.89 |
| 7 | IgPGK | 2.86 ± 0.64 | IgEF1ɑ | 5.77 ± 1.34 | IgEF1β | 4.26 ± 0.98 | IgEF1β | 4.38 ± 1.00 |
| 8 | IgUBQ | 5.22 ± 1.12 | IgUBQ | 6.92 ± 1.42 | IgUBQ | 4.46 ± 0.95 | IgUBQ | 5.23 ± 1.14 |