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. 2021 May 10;11:9865. doi: 10.1038/s41598-021-89312-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DN, but not SN, promotes indel formation in human iPSCs. (A) Schematic representation of two gRNA sequences (on two opposite strands) upstream of the CAG repeat in exon 7 of human PPP2R2B gene. Primers F1 and R1 were used for PCR amplification. (B) Indel frequencies calculated by TIDE using sequence traces. BCL-XL overexpression increased indels induced by DN but did not increase indels induced by SN. Mean and SEM. N = 3 independent experiments. One-way ANOVA F(3,8) = 150.0; p < 0.001; Tukey’s multiple comparison test ***p < 0.001.