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. 2021 May 10;11:9842. doi: 10.1038/s41598-021-89052-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Follicle growth in no injection and control groups. (a) The diameter of cultured follicles did not significantly differ between follicles in the no injection group and negative control siRNA-injected follicles in both mature (left) and premature (right) mice. The diameters of mature mice follicles were 470.4 ± 114.8 µm (n = 73) in the no injection group and 482.8 ± 111.3 µm (n = 74) in the control group. The diameters of premature mice follicles were 483.8 ± 81.99 µm (n = 75) in the no injection group and 457.5 ± 108.7 µm (n = 76) in the control group. (b) Representative pictures of follicles after 12 days of in vitro culture. (i) Mature mouse follicle of the no injection group, (ii) mature mouse follicle of the control group, (iii) premature mouse follicle of the no injection group, and (iv) premature mouse follicle of the control group. Scale bars, 100µm.