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. 2021 May 10;11:9846. doi: 10.1038/s41598-021-89128-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of the methylation changes observed at specific CpG sites across all the treatments and controls. The beta-values representing methylation levels are plotted at the vertical axis and the type of cell at the horizontal axis. Gain of methylation (hypermethylation) in the cells treated with BTZ and recovered from treatment was observed at CpG sites in (A) and (C), and loss of methylation at CpG sites in (B) and (D).