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. 2021 Apr 27;12:644662. doi: 10.3389/fmicb.2021.644662

TABLE 1.

A brief overview of the average summary statistics (i.e., number of samples, total raw read-pairs, average filtered/bar, average mapped/filtered) for each sample type of each library type (i.e., 16S amplicon libraries, 16S-cap enriched, and unenriched).

Library type Read length Sample type N samples Total raw read-pairs Total filtered reads Average filtered/Raw (Mean ± SD) Average mapped/Filtered (Mean ± SD)
Amplicon-16S/V3V4 PE300 Rat feces 5 318,561 247,781 (77.3 ± 6.2)% NA
Amplicon-16S/V3V4 PE300 BEI Mock 1 136,184 52,734 38.7% NA
Amplicon-16S/V3V4 PE300 Zymo Mock 1 49,828 24,301 48.8% NA
Amplicon-16S/V4 PE250 Mice feces 8 526,754 389,000 (77.6 ± 7.1)% NA
Enriched PE150 Mice feces 8 11,474,476 8,321,081 (70.1 ± 5.4)% (59.1 ± 0.8)%
Enriched PE150 Rat feces 5 9,470,428 6,450,541 (72.9 ± 2.1)% (57.8 ± 4.1)%
Enriched PE150 BEI Mock 1 8,203,396 5,345,638 76.7% 70.4%
Enriched PE150 Zymo Mock 1 5,140,030 3,359,376 76.5% 70.1%
Enriched PE300 Mice feces 8 1,573,122 1,050,608 (75.1 ± 3.2)% (59.9 ± 2.1)%
Enriched PE300 BEI Mock 1 1,108,481 737,309 75.2% 75.7%
Enriched PE300 Zymo Mock 1 721,740 467,250 77.2% 73.8%
Unenriched PE150 Mice feces 8 37,894,050 28,219,552 (68.6 ± 6.4)% 0.1%
Unenriched PE150 Rat feces 5 28,448,468 16,266,683 (87.4 ± 0.9)% 0.1%
Unenriched PE150 BEI Mock 1 8,889,636 6,263,379 71% 0.2%
Unenriched PE150 Zymo Mock 1 7,001,503 4,985,957 70.2% 0.2%