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. 2021 Apr 27;12:649010. doi: 10.3389/fmicb.2021.649010

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Copyright © 2021 Huang, Gu, Xue, Yu, Tang, Huang, Zhang and Jiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Feasibility assessment of the CRISPR-Cas12b-based C. jejuni detection system for onsite C. jejuni detection based on chicken samples contaminated with different doses of C. jejuni. (A) Chicken samples (25 g) detected with the CRISPR-Cas12b system; Tubes 1–10: crude DNA extracted from chicken samples contaminated with 10-fold gradient dilutions of C. jejuni NCTC81-176 with an initial concentration of OD₆₀₀ = 1; Tube PC: positive sample; Tube ND: no DNA and probe were added; Tube NC: only the 5′−⁶FAM-N12-3′-BHQ1 probe was added. (B) Fluorescence intensity corresponding to each tube in panel (A). Paired two-tailed t test, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. NS, Not significant. PC, Positive control. ND, Not detected. NC, Negative control.