Table 1.
Primers for candidate target and reference genes in expression studies by qPCR in the jejunum.
| Genes | Gene name | Size, pb | Annealing temperature, °C | Accession No. | Sequence (5′ to 3′) | Reference |
|---|---|---|---|---|---|---|
| ALAS1 | 5’-aminolevulinate synthase-1 | 70 | 60 | NM_001018012.1 | F: CAGGTGGACAGGAAAGGTAAAGA R: CAGCCTCAGTGTTCCTCACCAC |
This study |
| MYOM2 | Myomesin-2 | 167 | 60 | XM_015284819.2 | F: CACTTGCCACTGTCGTATGATG R: TCTCTATACCACTCAGCACGAGG |
This study |
| CD36 | CD36 molecule | 85 | 60 | XM_025147449.1 | F: CTGACATTTGCAGATCCATCTATGG R: CGAGGAACTGTGAAACGATACAGT |
This study |
| ALDH1A1 | Aldehyde dehydrogenase 1 family, member A1 | 191 | 60 | XM_015280233.2 | F: GCAGATGCAGACTTGGATGAAG R: CCTTGCTGTACACCAGGTAACAG |
This study |
| FABP1 | Fatty acid binding protein 1 | 86 | 60 | NM_204192.3 | F: GAAGGTAACAACAAACTGGTTGCT R: GTCATTGTATGGGTGATGGTGTC |
This study |
| FABP2 | Fatty acid binding protein 2 | 184 | 60 | NM_001007923.1 | F: GCAATGGGCGTGAATGTGA R: AGCCTGAAAGTTCAGTCCCGT |
This study |
| GCG | Glucagon | 144 | 60 | NM_205260.5 | F: CGGATCAGCTCTCAAGTAATGC R: CTCGTCCATTCACTAACCAAGC |
This study |
| ME1 | Malic enzyme 1 | 164 | 60 | NM_204303.1 | F: GCTGTCAAAAGGATATGGATGGT R: GCAGCGACTCCTATTAGCACA |
This study |
| PEX13 | Peroxisomal biogenesis factor 13 | 264 | 60 | NM_001199421.1 | F: TGGGAGAACCGGCGATTAGT R: CAAGCCACCGTATCCATAACTG |
This study |
| MSRB1 | Methionine sulfoxide reductase B1 | 164 | 60 | NM_001346746.2 | F: TCGAGCCGGGTGTGTATGTA R: CTTGCCACAGGACACCTTTAAG |
This study |
| PRKG2 | Protein kinase cGMP-dependent 2 | 161 | 60 | XM_025149790.1 | F: CGACATGCCAAAAGAAGATCC R: GTTCAACCCTTCCGAACCCA |
This study |
| ACOX2 | Acyl-CoA oxidase 2 | 163 | 60 | XM_004944647.3 | F: CACTGTGCCCAGGTATAACTGC R: GACCCACGCCTTACATAGGTG |
This study |
| ASBT | Solute carrier family 10 member 2 | 119 | 60 | NM_001319027.1 | F: GTGGGTTATCACACCTAAGTTATG R: CACTGTACGACATCTGCTCCAAG |
This study |
| ACOD1 | Aconitate decarboxylase 1 | 146 | 60 | XM_015275372.2 | F: AAGGTTTCACCCACCAACAGTG R: GAGTTGCTGCGTTAGCCAGTG |
This study |
| IL21R | Interleukin 21 receptor | 111 | 60 | XM_025155100.1 | F: CTGGGAGACTCAGAAGATCAAATC R: GGTCTGGCTCTCACTTGGAATTC |
This study |
| GZMA | Granzyme A | 56 | 60 | NM_204457.1 | F: CTTCCTGGAGATTTGTGCGTG R: GAGTGTGGTGCTACTTCATGTCCT |
This study |
| CHPT1 | Choline phosphotransferase 1 | 183 | 60 | XM_015285488.2 | F: CGAGCAGGCACCTTTTTGG R: GCTATGCAGGATCCAAGGACA |
This study |
| DUSP4 | Dual specificity phosphatase 4 | 155 | 60 | NM_204838.1 | F: ATCACAGCCCTGCTGAACGT R: CAGCACTCTTTCACTGAGTCGATG |
This study |
| BCL2A1 | BCL2 related protein A1 | 78 | 60 | NM_204866.1 | F: GGTGGCTGGGAAAACGGT R: TGGTACTCTCTGAACAAGGAAAGAAC |
This study |
| LAT1 | Neutral amino acid transporter | 70 | 60 | KT876067.1 | F: GATTGCAACGGGTGATGTGA R: CCCCACACCCACTTTTGTTT |
Gilbert et al. (2007) |
| ZAP70 | Zeta chain of T cell receptor associated protein kinase 70 kDa | 110 | 60 | NM_001321556.1 | F: GCTGGACCTACAGTTGGGAAGA R: CAATGCTGTAGTAGTAGGTGCGGA |
This study |
| TBP | TATA-box binding protein | 147 | 62 | NM_20510383127 | F-TAGCCCGATGATGCCGTAT R- GTTCCCTGTGTCGCTTGC |
Li et al. (2005) |
| SDHA | Succinate dehydrogenase complex flavoprotein subunit A | 74 | 60 | NM_00127739 | F-ATACGGGAAGGAAGGGGTTG R-TGCTGGGGTGGTAAATGGTG |
Barzegar Nafari (2019) |