Figure 4.

Literature reported and putative autoantigens are detected in SLE samples by PIWAS. (A) PIWAS results from a comparison of SLE samples to controls against the human proteome were prioritized using outlier sum false discovery rate (FDR) as a measure of significance (y-axis, see Methods). For visualization, proteins were laid out according to chromosome location. (B) Among the top set of 22 ranked proteins, 5 are established autoantigens (Smith family in red, others in blue). (C) Strength (y) and location (x) of PIWAS scores for the small nuclear ribonucleoprotein-associated proteins B and B’ within SLE (n=31, purple) vs. control (n=1,157, grey). A cohort of anti-Sm predicate positive patients (n=35, orange) were compared to the same controls to validate the signal obtained using SLE specimens with unknown anti-Sm serostatus.