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. 2021 May 6;28(5):863–876.e6. doi: 10.1016/j.stem.2021.01.003

Figure 2.

Figure 2

Resting NSCs increasingly contribute to the proliferative NSC pool with time

(A) Mice received EdU via drinking water for 2 weeks to label proliferating and resting NSCs, followed by a 20-h chase.

(B) Dormant NSCs that proliferated during the 20-h chase period were identified as EdU–Ki67+ NSCs.

(C) The activation rate of dormant NSCs, normalized to the total size of the NSC pool, decreased with age, indicating deepening quiescence of this population.

(D) The absolute numbers of EdU–Ki67+ cells also decreased with age.

(E) The contribution of dormant NSCs to the proliferative NSC pool decreased with age, whereas the contribution of resting NSCs increased despite the resting NSC pool remaining relatively small even at advanced ages.

Graphs show mean ± SEM. Dots represent individual mice in (C) and (D); in (E), 3 mice were analyzed per time point. Statistics: one-way ANOVA in (C) and (D). Scale bar in (B), 10 μm.