Figure 6.

Ascl1 protein levels decrease with time and cause progressive changes in NSC behavior

(A) ASCL1-VENUS staining in hippocampal NSCs from 1-month-old mice (arrowheads indicate positive cells) using an anti-GFP antibody.

(B) ASCL1-VENUS staining in NSCs from 6-month-old mice.

(C) Fewer NSCs are positive for ASCL1-VENUS with age.

(D) The expression intensity of the ASCL1-VENUS protein also decreases with age.

(E) Ascl1^neo/neo and control mice were given EdU to label proliferating and resting NSCs and were culled following a 20-h chase.

(F) Hippocampal NSCs were returning more to quiescence after proliferating (EdU+Ki67−) in Ascl1^neo/neo mice than in controls.

(G) Dormant NSCs activated less frequently in Ascl1^neo/neo mice.

(H) Resting NSCs contributed more to the proliferative NSC pool in Ascl1^neo/neo mice than in controls.

(I) Ascl1^neo/neo mice had more NSCs than controls.

Graphs represent the mean ± SEM. Dots represent individual mice, except in (D), where dots represent individual cells (minimum of 20 cells analyzed per mouse, 2 mice per age). Statistics: one-way ANOVA in (C) and t test in (D) and (F)–(I). ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bar (located in A): 21.9 μm in (A) and (B). a.u., arbitrary units.