Figure 7.

Expression and activity of Huwe1 increases with time

(A) Ascl1 transcript levels in NSCs are largely unchanged between young and adult mice (scRNA-seq).

(B) The expression of the ubiquitin-ligase Huwe1 increases in older mice (scRNA-seq).

(C) 1- and 6-month-old Huwe1^fl/y and control mice were culled 1 week after receiving tamoxifen.

(D) The fold change increase in ASCL1+ NSCs in Huwe1^fl/y mice (relative to controls) was larger in the older cohort.

(E) The fold change increase in Ki67+ NSCs in Huwe1^fl/y mice (relative to controls) was also larger in the older cohort.

(F) Images of control and Huwe1^fl/y mice at 1 month of age; arrows indicate ASCL1+ NSCs.

(G) Images of control and Huwe1^fl/y mice at 6 months of age; arrows indicate ASCL1+ NSCs.

Graphs represent mean ± SEM or are violin plots in (A) and (B). Dots represent single cells in (A) and (B) and individual mice in (D) and (E). Statistics: t test performed on Pearson’s residuals with FDR-corrected p value in (A) and (B) and t test in (D) and (E). ^∗p < 0.05, ^∗∗p < 0.01. Scale bar (located in G): 28 μm in (F) and (G).