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. 2021 May 10;4:545. doi: 10.1038/s42003-021-02062-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A RNA editing quantified by RNAseq at the intended target site in three independent samples of bacteria expressing dsn3PLS-DYW, dsn3PLS-DYW together with MORF2 or dsn3PLS-DYW(E70A), together with MORF2. B Potential editing sites plotted by their predicted binding to dsn3PLS-DYW (x-axis) against the ratio of editing activity compared to the negative control (dsn3PLS-DYW(E70A) together with MORF2), expressed as the log of the odds ratio. Predicted binding scores were calculated with PPRmatcher (https://github.com/ian-small/PPRmatcher). The only significant site where the log(odds ratio) exceeds 2 is the intended target site, rpoA-78691, highlighted in red. The ~492,000 sites below this threshold are indicated by the density contours (grey-black). C Additional tests of editing in E. coli. From left to right: dsn3PLS-DYW(E70A) + MORF2 as a negative control; MORF2 and MORF9 enhance editing by dsn3PLS-DYW by similar amounts; a U at the rpoA-78691 editing position is not detectably reverse-edited to C; the clpP1-69942 site is not detectably edited with or without MORF2. All experiments were repeated in triplicate, as shown.