Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 10;4:540. doi: 10.1038/s42003-021-02071-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Effect of STAT3 activator IL-6 (50 ng/mL) on the expression of STAT3. Q-PCR and Western blot analysis of the relative expression of STAT3 mRNA and protein after IL-6 treatment in the indicated time points. b Western blot of nuclear and cytoplasmic distribution of total STAT3 and pSTAT3 after the treatment of IL-6 in the indicated time points. c Immunofluorescence analysis of STAT3 expression after IL-6 treatment in the indicated time points. Scale bar: 25 μm. d Q-PCR and Western blot analysis of STAT3 expression in control and two FMRP-knockdown HCCLM3 cells. e Immunofluorescence analysis of STAT3 in control and FMRP-overexpressing HCCLM3 cells. Scale bar: 50 μm. f Western blot analysis of STAT3 expression in HCCLM3-Control and HCCLM3-sh-FMRP#1 cells treated with 100 μg/mL cycloheximide (CHX) and/or IL-6 (50 ng/mL). g Western blot analysis of the effects of wild-type FMRP (WT) and mutated FMRP on the IL-6-mediated STAT3 translation. IL-6 failed to enhance the expression of STAT3 when the S114 was mutated. The right panel is the quantification of the intensity relative to β-actin. The values in the graphs represent the mean of three biologically independent experiments. Error bars represent ±s.d. *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed Student’s t-test.