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. 2021 May 10;12:2577. doi: 10.1038/s41467-021-22881-y

Fig. 7. Identification of hook and mesh basement membranes.

Fig. 7

a Immunofluorescence labelling of dorsal telogen HFs. Both laminin α5 (green) and perlecan (red) are detected in a hook-shaped basement membrane (BM; arrows) extending from the interface BM (open arrowheads). Perlecan also forms a mesh-like BM (filled arrowheads) within the dermal papilla (DP). Dashed line indicates the epidermal–dermal boundary. b Immunolocalizations of major BM proteins (green) in the hook and mesh BMs of dorsal telogen HFs. Nuclei were stained with DAPI (blue). c Graphical representation of the regional BM compositions in the hair germ (HG)–DP unit. Upper left-hand panel depicts the distinct BM structures. Other panels schematically summarize deposition patterns of BM components examined in (a, b) and Supplementary Fig. 5. d Close localization of dermal integrins with laminin α5-containing hook and interface BMs. Integrin α6 (upper panels, green) or β1 (lower panels, green) was co-immunostained with laminin α5 (red). Insets are magnified views of the dotted squares. Arrows and open arrowheads indicate the hook BM and interface BM, respectively. Scale bars: 5 μm (a, b), 3 μm (d), 1.5 μm (insets in (d)).