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The SERA assay workflow. In step 1, serum antibodies interact with antigen mimics displayed on the surface of bacteria allowing for capture of those bacteria. In step 2, the plasmid encoding the peptides that serve as the antigen mimics are amplified from the plasmids, and sample-specific sets are barcoded for NGS. In step 3, motif epitopes are identified that represent antigens for disease-specific antibodies.
