FIG 2.

Algorithm for Shigella flexneri serotype identification with real-time PCR assays. Each circle indicates one PCR target and each rectangle indicates one Shigella flexneri serotype, while each straight line links the required PCR targets to the relevant serotype, i.e., the PCR targets in the circles touching a straight line all have to be present to call the serotype at the end of that line. The shaded symbols indicate the first multiplex panel, and the white symbols indicate the second panel.