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. 2021 Apr 27;9:667800. doi: 10.3389/fcell.2021.667800

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Copyright © 2021 Liu, Han, Hu, Cai, Yang, Ren, Zhao, Lu, Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Circulating exosomal miRNAs exerted diagnostic and prognostic value in DLBCL. (A) TEM image of exosome particles shows typical cup-shaped morphology. (B) Western blot analysis of plasma exosomes with exosomal biomarker Tsg101 and CD9. (C–E) QRT-PCR analysis of the expression levels of exosomal miR-107, miR-375-3p, and miR-485-3p in the plasma of DLBCL (n = 42) and healthy controls (n = 31). (F) Receiver operating characteristic curves of biomarkers. (G) Alluvial diagram of miR-107 expression in groups with different clinical characteristics. Red represented high expression of miR-107; blue represented low expression of miR-107. (H) Kaplan–Meier analysis of DLBCL patients from Shandong Provincial Hospital. (I) Prognosis of miRNAs in various kinds of cancers from miRNAcancerMap database. Data are mean ± s.d., n ≥ 3. *p < 0.05, **p < 0.01, and ***p < 0.001. TEM, transmission electron microscopy. ROC, receiver operating characteristics.