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. 2021 Apr 27;9:667800. doi: 10.3389/fcell.2021.667800

FIGURE 6.

FIGURE 6

MiR-107 inhibited DLBCL tumorigenesis through targeting 14-3-3η. (A,B) Western blot (A) and qRT-PCR analysis (B) of 14-3-3η expression in DLBCL cell lines. Data were normalized by GAPDH. In the Western blot assay, the ratio of 14-3-3η/GAPDH is shown below the bands. (C) QRT-PCR analysis of 14-3-3η in the plasma exosomes of DLBCL patients. (D) Analysis at confocal microscopy of 14-3-3η expression in the biopsy tissues of DLBCL patients. (E,F) Western bolt (E) and qRT-PCR analysis (F) of 14-3-3η expression in OCI-LY3 cells transfected with miR-107 Agomir and OCI-LY8 cells transfected with miR-107 Antagomir. (G) 14-3-3η binding site of the WT and mutated type with miR-107. (H) Co-transfection of HEK293T cells with WT or Mutant (MUT) 14-3-3η 3’-UTR and miR-107 Agomir, as well as miR-107 normal control (miR-107 NC). The relative luciferase activity of HEK293T cells were determined. (I) Mechanism diagram of 14-3-3η regulation of FOXO1 and BAD in KEGG. (J) Expression of miR-107 target genes in GEPIA database (Normal = 337, DLBCL = 47).