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. 2021 Feb 16;104(5):1045–1057. doi: 10.1093/biolre/ioab020

Figure 2.

Figure 2

Conditional deletion of Cripto in the mouse uterus. The Cre-recombinase is driven under the progesterone receptor (PR) promoter, thereby creating a specific deletion of Cripto in reproductive tissues of mice bearing loxP insertions before exon 3 after exon 5 of Cripto gene (A). By IHC, Cripto deletion was confirmed in the implantation sites where Cripto localization (brown) is expected in luminal epithelium and stromal cells on d3.5pc (B-C′) and in decidual cells and differentiating stromal cells on d5.5pc (D-E′). In B′, although there is no sign of Cripto expression in the uterus, the localization of Cripto in the embryo present within the uterine lumen is quite evident which further confirms the successful deletion if Cripto in the maternal tissues. B′, C′, D′, and E′ are higher magnification of the fields defined by black boxes in B, C, D, and E respectively. Images are representative of ≥3 independent samples for each timepoint. Negative control sections subjected to dilution buffer with no primary antibody displayed no positive staining (images not presented) AM, antimesometrial pole; De, decidual cells; Em, embryo; GE, glandular epithelium; LE, luminal epithelium; Lu, lumen; M, mesometrial pole; My, myometrium; St, Stroma. (Scale bar: B, C, D, and E: 250 μm; B′ and C′: 50 μm; D′ and E′: 25 μm).