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. 2021 Apr 27;9:651583. doi: 10.3389/fcell.2021.651583

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Copyright © 2021 Ning, Zhang, Zhang, Zhu, Ding, Jiang, Zhang, Luo and Qin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of decellularization effectiveness. (A) H&E staining and DAPI staining of the NTSs and DTSs. Scale bar = 200 μm. (B) PicoGreen analysis of residual DNA content in the NTSs and DTSs. *Signifies a P-value of <0.05 as compared to the NTSs.