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. 2021 Apr 27;9:651583. doi: 10.3389/fcell.2021.651583

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Copyright © 2021 Ning, Zhang, Zhang, Zhu, Ding, Jiang, Zhang, Luo and Qin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxicity analysis of the C-gel and T-gel. (A) Live/Dead staining of NIH 3T3 fibroblasts seeded on the C-gel and T-gel at 1, 3, and 5 days by fluorescence microscopy (green, live cells; red, dead cells). Scale bar = 500 μm. (B) The cytotoxicity of the C-gel and T-gel using NIH-3T3 fibroblasts cultured for 1, 3, and 5 days as determined by the CCK-8 assay. *Signifies a P-value of<0.05 as compared to the C-gel; #, signifies a P-value of <0.05 as compared to 1 day for each gel; △, signifies a P-value of <0.05 as compared to 3 days for each gel.