Figure 4.

Avidity of FH autoantibodies. Avidity of the FH autoantibodies was determined by ELISA using NaSCN as a chaotropic agent. (A) Avidity profile of the autoantibodies. Residual binding of the FH autoantibodies from serum was measured after applying various NaSCN concentrations. Data are normalized to binding in the absence of NaSCN (=100% binding). A representative experiment out of two is shown. (B) Avidity indexes calculated at 0.5 M NaSCN. Avidity index is calculated as the ratio of remaining bound autoantibody after NaSCN elution (AU/ml)/autoantibody bound without NaSCN elution (AU/ml). Data are means ± SD of three measurements.