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Epitope mapping using mutant FH19-20 fragments. The wild type FH19-20 fragment and 14 mutants containing single amino acid exchanges were immobilized in microtiter plate wells and incubated with patient serum. Autoantibody binding was detected using HRP-conjugated anti-human IgG. Data are mean ± SD of three experiments. *p < 0.05 and ***p < 0.001, one-way ANOVA.
