Figure 8.

Autoantibody binding to reduced FH. Microplate wells were coated with equimolar amounts of HSA, FH and TCEP-treated, reduced FH, and after blocking, incubated with patients’ sera diluted 1:50 in PBS. Autoantibody binding was detected by HRP-conjugated anti-human IgG. As controls, sera of two FH autoantibody-negative NMO patients (#176 and #193), an aHUS patient and a healthy control (normal human serum, NHS) were used. Goat antiserum was used to prove that FH and reduced FH were immobilized on the plate. Data are mean ± SD of two experiments.