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Inhibition of C3b binding to FH19-20 by FH autoantibodies. The immobilized FH19-20 fragment was incubated with IgG purified from the sera of NMOSD patients, healthy human sera (C) or serum of an aHUS patient with FH autoantibodies, then C3b was added. C3b binding was detected using HRP-conjugated anti-human C3 antibodies. Data are normalized to binding in the absence of IgG (=100%) and are means + SD of three measurements.
