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. 2021 Apr 27;8:554190. doi: 10.3389/fcell.2020.554190

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Copyright © 2021 Chen, Hu, Han, Zhu, Fan, Chen, Li and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Content of estradiol and expression levels of ERβ and ERα in human and mouse endometriosis. (A) The expression of estradiol in human and mouse endometriosis/normal tissues determined by ELISA. (B) The mRNA expression of ERα in human and mouse endometriosis/normal tissues determined by RT-qPCR. (C) The mRNA expression of ERβ in human and mouse endometriosis/normal tissues determined by RT-qPCR. (D) The relative expression of ERβ/ERα in human and mouse endometriosis/normal tissues determined by RT-qPCR. (E) The protein expression of ERα and ERβ in human and mouse endometriosis/normal tissues determined by Western blot analysis. (F) The protein expression of ERβ/ERα in human and mouse endometriosis/normal tissues determined by Western blot analysis. (G), The statistical analysis of immunohistochemical staining of ERα and ERβ in human and mouse endometriosis/normal tissues. (H) HE staining of mouse endometriosis and normal tissues. (I), The statistical analysis of fluorescence staining of Ki67 in human and mouse endometriosis/normal tissues. (J) The statistical analysis of immunohistochemical staining of cleaved caspase-8 in human and mouse endometriosis/normal tissues. Comparisons between two groups were analyzed using independent sample t-test. *p < 0.05 vs. the human or mouse normal tissues. n = 78 for human normal tissues; n = 91 for human endometriosis tissues; n = 14 for mouse endometriosis/normal tissues.