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. 2021 Apr 27;8:554190. doi: 10.3389/fcell.2020.554190

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Copyright © 2021 Chen, Hu, Han, Zhu, Fan, Chen, Li and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ERα was down-regulated and ERβ was up-regulated in endometriosis-related HESCs in vitro. (A) The expression of estradiol in HESCs-NC and HESCs-En determined by ELISA. (B) The mRNA expression of ERα in HESCs-NC and HESCs-En determined by RT-qPCR. (C) The mRNA expression of ERβ in HESCs-NC and HESCs-En determined by RT-qPCR. (D) The relative expression of ERβ/ERα in HESCs-NC and HESCs-En determined by RT-qPCR. (E) The protein expression of ERα and ERβ in HESCs-NC and HESCs-En determined by Western blot analysis. (F) The protein expression of ERβ/ERα in HESCs-NC and HESCs-En determined by Western blot analysis. Comparisons between two groups were analyzed using independent sample t-test. *p < 0.05 vs. the HESCs-NC cells. Cell experiments were conducted in triplicate.