Skip to main content
. 2021 Apr 27;8:554190. doi: 10.3389/fcell.2020.554190

FIGURE 4.

FIGURE 4

BAG2 inhibits CHIP-mediated degradation of ERβ in HESCs in vitro. (A) The mRNA expression of BAG2 in human and mouse endometriosis/normal tissues and HESCs determined by RT-qPCR. (B) The protein expression of BAG2 in human and mouse endometriosis/normal tissues and HESCs determined by Western blot analysis. (C) The statistical analysis of immunohistochemical staining of BAG2 in human and mouse endometriosis/normal tissues. (D) The interaction between CHIP and BAG2 by Co-IP assay. (E) Ubiquitin of ERβ in the presence or absence of BAG2 by ubiquitin test. (F) The protein expression of ERβ in the presence or absence of BAG2 by Western blot analysis. (G) The statistical analysis of panel (F). Comparisons between two groups were analyzed using independent sample t-test. *p < 0.05 vs. human or mouse normal tissues, HESCs-NC cells or the absence of BAG2. n = 78 for human normal tissues; n = 91 for human endometriosis tissues; n = 14 for mouse endometriosis/normal tissues. Cell experiments were conducted in triplicate.