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. 2021 Apr 27;8:554190. doi: 10.3389/fcell.2020.554190

FIGURE 7.

FIGURE 7

The interference of BAG2 and MDM2 showed therapeutic effects on mouse endometriosis. Con-M mice were treated with overexpressed MDM2 and BAG2 using oe-NC as control, while En-M mice were treated using sh-BAG2 and sh-MDM2 with sh-NC as control. (A) The mRNA expression of BAG2 and MDM2 determined by RT-qPCR. (B) The protein expression of BAG2 and MDM2 determined by Western blot analysis. (C) The protein expression of ERα determined by Western blot analysis. (D) The protein expression of ERβ determined by Western blot analysis. (E) The protein expression of ERβ/ERα determined by Western blot analysis. (F) Fluorescence staining of Ki67 in mouse tissues. G, The statistical analysis of (F). (H) Immunohistochemical staining of cleaved caspase 8 in mouse tissues. (I) The statistical analysis of panel (H). Comparisons between two groups were analyzed using independent sample t-test. n = 8. *p < 0.05 vs. oe-NC treatment; #p < 0.05 vs. sh-NC treatment.