Figure 6.

Pluronic acid and probenecid enhanced carbachol-activated M3-AChR-induced calcium signal. (A) Fura-2/AM was loaded in DMEM the presence or absence of pluronic acid and the cells were then tested for vehicle-induced stretch-activated calcium response and carbachol (10⁻⁴ M) -induced calcium signal. (B) Probenecid (2.5 mM) was present or not present during Fura-2/AM loading and esterase cleavage and then the cells were then tested for vehicle-induced stretch-activated calcium response and carbachol (10⁻⁴ M) -induced calcium signal. Data shown as mean ± s.e.m. are representative of three independent experiments. Data for all experiments are shown in Supplementary Fig. 4A, B, C and D.