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. 2021 May 7;218(7):e20202699. doi: 10.1084/jem.20202699

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© 2021 Cotton et al.

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Figure 3. — Loading and structural elucidation of CD1a–SM complexes with altered fatty acyl chain length. (A) Deglycosylated CD1a carrying HEK293T cell–derived lipids (endo) was treated with CHAPS detergent (mock) or different species of SM and resolved on isoelectric focusing gels. In case of 36:2 SM, endogenous lipids were “washed out” using 0.5% tyloxapol before SM loading. Results are typical for two or more experiments. (B) 2Fo-Fc electron density maps of the ligands in the cleft are contoured at σ of 0.7 Å. (C) Overview of the binary structures of CD1a/β2m heterodimer (gray and blue, respectively) bound to 42:1 SM (orange), 42:2 SM (teal), or 36:2 SM (purple).