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. 2021 May 7;218(7):e20202699. doi: 10.1084/jem.20202699

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© 2021 Cotton et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure S1. — Detailed SM profiles from HEK293 cells. CD1a proteins generated in HEK293S or HEK293T.TPM cells were treated with chloroform and methanol, with eluents subject to analysis by positive ion mode HPLC-MS, where the combined length (X) and unsaturations (Y), shown as X:Y, were deduced based on the detected m/z values of natural SMs nearly coeluting with an SM standard. CD1a constructs and proteins were generated at the NIH tetramer facility or at Monash University (Monash).