Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 7;218(7):e20202699. doi: 10.1084/jem.20202699

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Cotton et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S2. — Total lipid extracts from the indicated sources were analyzed by HPLC-MS in the positive ion mode. (A and B) SM ion (red) intensity values were matched to the deduced SM variant (A), where the overall chain length (CH2 units) and unsaturations present in both chains are indicated in (B). (C) MS/MS/MS of 34:1 SM from CD1a eluents and an authentic standard detected ion corresponding to the alkyl chains present in the sphingosine base (blue) and the fatty acyl unit (red), allowing assignment of a C16 fatty acid (m/z 280.4) as the predominant species. Thus, most chain-length variations in SMs are accounted for by the fatty acyl unit. Unsat, number of unsaturations in both chains.