Figure 1.

PLCβ4 is expressed in CD4⁺ and CD8⁺ T cells. (A) Quantitative PCR analysis of the levels of Plcb4 mRNA in the indicated cells. (B) Quantitative PCR analysis of the levels of Plcb4 mRNA in the indicated thymocytes and splenic CD8⁺ T cells. (C) Western blot analysis of PLCβ4 proteins in the indicated thymocytes and splenic CD8⁺ T cells. (D) Purified CD4⁺ or CD8⁺ T cells (2 × 10⁶ cells each) from WT or PLCβ4-deficient mice were lysed. PLCβ4 protein levels in the indicated lysates were determined by Western blotting with indicated Abs. (E) PLCβ4 protein levels in the indicated organs from WT or PLCβ4-deficient mice were determined by Western blotting with indicated Abs. Indicated values are means ± SD of three biological replicates (A). Data are representative two independent experiments (C–E) or cumulative of three independent experiments (B).