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. 2021 May 10;218(7):e20201763. doi: 10.1084/jem.20201763

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© 2021 Sasai et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure S1. — Generation of PLCβ4-deficient mice. (A) The gene-targeting strategy for Plcb4 locus using ES cells. (B) Southern blot analysis of total genomic DNA extracted from the WT or PLCβ4^+/− ES cells. Genomic DNA was digested with EcoRI, electrophoresed, and hybridized with the radiolabeled probe indicated in (A). Southern blotting resulted in an 8-kb band for WT locus and a 2-kb band for mutated locus. Data are representative two independent experiments (B).