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. 2021 May 10;218(7):e20201763. doi: 10.1084/jem.20201763

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© 2021 Sasai et al.

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Figure S2. — PLCβ4-deficient mice show intact T cell development and Th1 differentiation. (A) Percentages and cell numbers of CD4⁻/CD8⁻, CD4⁺/CD8⁻, CD4⁻/CD8⁺, and CD4⁺/CD8⁺ in the thymocytes from WT or PLCβ4-deficient mice were analyzed by flow cytometry. (B) Percentages and cell numbers of CD4⁺ and CD8⁺ T cells in the splenocytes from WT or PLCβ4-deficient mice were analyzed by flow cytometry. (C) Naive CD4⁺ T cells from WT or PLCβ4-deficient mice were cultured under Th1-polarizing conditions for 3 d. Frequencies of IFN-γ producers were determined by intracellular staining using flow cytometry. Indicated values are means ± SD of three biological replicates (A–C). N.S., nonsignificant.