Figure S5.

Generation of T cell– or myeloid-specific PLCβ4-deficient mice. (A and B) BMDM, CD4⁺, or CD8⁺ T cells from WT, PLC4β4^fl/fl, Lyz2-Cre/PLCβ4^fl/fl, or Lck-Cre/PLCβ4^fl/fl mice were lysed. PLCβ4 protein levels in the indicated lysates were determined by Western blotting with indicated Abs. (C) WT or PLCβ4-deficient mice (n = 3 per group) were infected with 1 × 10⁵ T. gondii. After 5 d, the percentages of CD69⁺ (left) and CD62L^low (right) population on CD4⁺ T cells in the spleens from parasites infected WT or PLCβ4-deficient mice were measured by flow cytometry. (D) WT or PLCβ4-deficient mice (n = 6 per group) were infected with 1 × 10⁵ OVA expressing irradiated T. gondii. 14 d after infection, the percentages and cell numbers of OVA tetramer-positive CD4⁺ T cells in the spleens from parasite-infected WT or PLCβ4-deficient mice were measured by flow cytometry. (E) Luciferase activities of serial dilutions of Pru expressing luciferase (10³–10⁷) were plotted. A standard curve of luciferase activity (x axis) and parasite number (y axis) was generated by the data plots. Indicated values are means ± SD of three biological replicates (C). N.S., nonsignificant. Data are cumulative of three independent experiments (D) and representative of two (A, B) or three (E) independent experiments.