FIGURE 3.

Cultured tumor-infiltrating lymphocyte (TIL) (pre-rapid expansion protocol) from soft tissue sarcoma using digest method. A, TIL can be expanded from soft tissue sarcoma using the digest method to a clinically meaningful degree. B, The phenotype of primary TIL cultures in matched pairs derived the from digest method is similar to the fragment method. C, Data regarding the expansion of lymphocytes from the fresh tumor are presented for individual samples to demonstrate linked differences between digest and fragment method of TIL culture. DDLPS indicates dedifferentiated liposarcoma; DSRCT, desmoplastic small round cell tumor; GIST, gastrointestinal stromal tumor;LMS, leiomyosarcoma; MPNST, malignant peripheral nerve sheath tumor; NS, nonsignificant; UPS, undifferentiated pleomorphic sarcoma; WDLPS, well-differentiated liposarcoma.