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. 2021 Apr 23;12:658372. doi: 10.3389/fimmu.2021.658372

Figure 3.

Figure 3

Comparing SCD, IP, and sIP MS dataset intersections. (A) Venn comparisons of the intersections of the IP five experimental replicate union dataset (red) and unions of three single-replicate sIP (green) or SCD (blue) single-allele (A3, B7, C7) datasets. Overlap is 45% between the IP and SCD datasets, corresponding to observation of 63% of the IP peptides in the SCD dataset. The overlap is 25% between IP and sIP datasets. (B) Venn comparisons of the intersections of the IP five experimental replicate union dataset (red) and the union of the available multi-replicate SCD (blue) single-allele (A3, B7, C7) datasets. 80% of the IP peptides were observed in the SCD replicate-union dataset. Inset: Peptides in each of the three Venn domains were analyzed for binding with NetMHCpan 4.1; results are plotted as relative percentage “strong binder” (green), “weak binder” (yellow), or “non-binder” (orange), summed over lengths and the three alleles in the analysis. (C) Venn comparisons of the intersections of A3, B7, or C7 sIP/SCD single-run datasets. Overlaps are 28% (A3), 24% (B7), and 27% (C7). Venn diagrams were generated with BioVenn (33).