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. 2021 May 1;2(2):100489. doi: 10.1016/j.xpro.2021.100489

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Characterization of the heart and liver EC sample purity

(A) t-SNE visualization of scRNA-seq analyses on ECs isolated from mouse heart showing representative EC and non-EC gene markers expression and t-SNE visualization of the number of genes expressed per cell. Red arrowheads are pointing at cells highly expressing the marker gene (or cells with low gene number expression). Color scale: red, high expression; blue, low expression.

(B) Top: t-SNE visualization of heart (non-) ECs color coded per cell type. Red arrowheads are pointing at non-ECs. Bottom: bar plot illustrating the quantification of EC and non-ECs.

(C) t-SNE visualization of scRNA-seq analyses on ECs isolated from mouse liver showing representative EC and non-EC gene markers expression and t-SNE visualization of the number of genes expressed per cell. Red arrowheads are pointing at cells highly expressing the marker gene (or cells with low gene number expression). Color scale: red, high expression; blue, low expression.

(D) Top: t-SNE visualization of liver (non-) ECs color coded per cell type. Red arrowheads are pointing at non-ECs. Bottom: bar plot illustrating the quantification of EC and non-ECs