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. 2021 May 1;2(2):100489. doi: 10.1016/j.xpro.2021.100489
Reagent Final concentration Amount
KnockOutTM DMEM-medium - 473 mL
Penicillin/Streptomycin 1% (v/v) 5 mL
Antibiotic-Antimycotic 2× 10 mL
Sodium Pyruvate 1 mM 5 mL
MEM Non-Essential Amino Acids Solution 1× 5 mL
Endothelial Cell Growth Factor supplements (ECGS/ Heparin) 1× 2 mL (one vial)