| Reagent | Final concentration | Amount |
|---|---|---|
| HBSS (Ca2+, Mg2+) | - | 475 mL |
| DTT | 1.5 mM | 0.23 g |
| FBS | 5% (v/v) | 25 mL |
| EDTA | 0.5 mM | 0.5 mL (from 5 M pre-prepared stock solution according to manufacturer's instructions) |
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