| Reagent | Final concentration | Amount |
|---|---|---|
| Supplemented KnockOutTM DMEM-medium | - | 7.425 mL |
| Collagenase I | 0.1% (w/v) | 10 mg |
| Collagenase IV | 0.25% (w/v) | 25 mg |
| Dispase | 0.625 U/mL | 2.5 mL (from 2.5 U/mL stock solution) |
| DNAse I | 7.5 μg/mL | 75 μL (from 1 mg/mL stock solution) |
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